Dystrophin anticorps

N° du produit ABIN6657490

N° du produit (Fournisseur): 200-301-p36

L’anticorps Souris Monoclonal anti-Dystrophin a été validé pour IHC, WB et ELISA. Il convient pour détecter Dystrophin dans des échantillons de Humain.

Aperçu rapide pour Dystrophin anticorps (ABIN6657490)

Antigène: Dystrophin (DMD)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dystrophin est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: MANDYS8

Détail du produit anti-Dystrophin anticorps

N° du produit (Fournisseur): 200-301-p36

Fournisseur: Rockland

Fonction: Dystrophin Antibody

Réactivité croisée (Details): This product reacts with human, mouse, rat, and rabbit.

Purification: Anti-Dystrophin antibody was purified from mouse ascites by protein A chromatography.

Immunogène: Dystrophin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to human dystrophin fragment.

Isotype: IgG2b

Information d'application

Indications d'application:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 1-2 μg/mL

Commentaires:

Anti-Dystrophin is tested for Immunohistochemistry -F and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~425 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 200μL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Dystrophin

Antigène: Dystrophin (DMD)

Autre désignation: Dystrophin

Sujet:

Synonyms: BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272

Background: Dystrophin(DMD) gene has 79 exons spanning at least 2,300 kb (2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons, the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects. This antibody is suitable for researchers interested in Muscular Dystrophies and Dystrophin-Glycoprotein Complexes and cardiovascular research.

Gene Name: DMD

ID gène: 1756

NCBI Accession: NP_000100

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development