CD34 anticorps (C-Term)
Aperçu rapide pour CD34 anticorps (C-Term) (ABIN6657527)
Antigène
Voir toutes CD34 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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N° du produit (Fournisseur)
- 600-401-p90
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Fournisseur
- Rockland
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Fonction
- CD34 Antibody
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Réactivité croisée (Details)
- Anti-CD34 antibody is directed against human CD34 protein.
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Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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Immunogène
- CD34 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human CD34.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry_Dilution: 0.5-1 μg/mL
Flow_Cytometry_Dilution: 1-3 μg/1x106 cells
Western_Blot_Dilution: 0.1-0.5 μg/mL
Other: Immunocytochemistry: 0.5-1 μg/mL
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Commentaires
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Anti-CD34 is tested for Flow Cytometry, Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~40.7 kDa corresponding to the appropriate cell lysate or extract.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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Buffer
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Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide
Stabilizer: 5 mg BSA
Preservative: 0.05 mg Thimerosal -
Agent conservateur
- Thimerosal (Merthiolate)
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Précaution d'utilisation
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- CD34
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Autre désignation
- CD34
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Sujet
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Synonyms: Hematopoietic progenitor cell antigen CD34, CD34 antibody
Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis. This antibody is suitable for researchers interested in stem cell, cancer and neuroscience research.
Gene Name: CD34
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ID gène
- 947
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NCBI Accession
- NP_001020280
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UniProt
- P28906
Antigène
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