Lamin B1 anticorps (C-Term)

N° du produit ABIN6657537

N° du produit (Fournisseur): 600-401-p62

Cet anticorps Lapin Polyclonal détecte spécifiquement Lamin B1 dans WB et IHC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Lamin B1 anticorps (C-Term) (ABIN6657537)

Antigène: Lamin B1 (LMNB1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Lamin B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Lamin B1 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-p62

Fournisseur: Rockland

Fonction: Lamin B1 Antibody

Réactivité croisée (Details): Anti-Lamin B1 antibody is directed against human Lamin B1 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogène: Lamin B1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Commentaires:

Anti-Lamin B1 is tested for Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~66.4 kDa corresponding to the appropriate cell lysate or extract. WB tested in Human, Mouse, Rat, IHC-P (heat) and IHC-F in human and rat, ICC in human, and ICC/IF in human.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Lamin B1 (ABIN6657537)

Mastej, Axen, Wary, Minshall, Wary: "A requirement for Krüppel Like Factor-4 in the maintenance of endothelial cell quiescence." dans: Frontiers in cell and developmental biology, Vol. 10, pp. 1003028, (2022) (PubMed).

Détails sur Lamin B1

Antigène: Lamin B1 (LMNB1)

Autre désignation: Lamin B1

Sujet:

Synonyms: LMNB1, LMN2, LMNB, Lamin B1 antibody

Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This antibody is suitable for researchers interested in chromatin antibody research.

Gene Name: LMNB1

ID gène: 4001

NCBI Accession: NP_001172028.11

UniProt: P20700

Pathways: Apoptose, Caspase Cascade in Apoptosis