CD80 anticorps (FITC)

N° du produit ABIN6657768

N° du produit (Fournisseur): 200-b02-n89

Cet anticorps Hamster Monoclonal détecte spécifiquement CD80 dans FACS, IHC, IP et FM. Il présente une réactivité envers Souris.

Aperçu rapide pour CD80 anticorps (FITC) (ABIN6657768)

Antigène: CD80

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CD80 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: 16-10A1

Détail du produit anti-CD80 anticorps (FITC)

N° du produit (Fournisseur): 200-b02-n89

Fournisseur: Rockland

Fonction: CD80 Fluorescein Antibody

Réactivité croisée (Details): Cross reactivity with CD80 from other sources has not been tested.

Purification: Fluorescein conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80.

Stérilité: Sterile filtered

Immunogène: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.

Isotype: IgG

Labeling Ratio: 2-8

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Other: Sufficient to run approximately 500 tests

Commentaires:

Anti-CD80 is tested for FLOW and useful for Immunohistochemistry, Immunofluorescence, and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Date de péremption: 6 months

Détails sur CD80

Antigène: CD80

Autre désignation: CD80

Sujet:

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Background: Anti-CD80 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Gene Name: Cd80

ID gène: 12519

NCBI Accession: NP_033985

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints