STAT4 anticorps (AA 730-749)

N° du produit ABIN6657793

N° du produit (Fournisseur): 100-401-861

L’anticorps Lapin Polyclonal anti-STAT4 a été validé pour WB, ELISA et IP. Il convient pour détecter STAT4 dans des échantillons de Souris.

Aperçu rapide pour STAT4 anticorps (AA 730-749) (ABIN6657793)

Antigène: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-STAT4 anticorps

Épitope: AA 730-749

N° du produit (Fournisseur): 100-401-861

Fournisseur: Rockland

Fonction: Stat4 Antibody

Réactivité croisée (Details):  Reactivity occurs against mouse STAT4 protein.

Purification: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 725-749 of Mouse STAT-4.

Isotype: IgG

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: Use 5 μL

ELISA_Dilution: 1:5,000 - 1:25,000

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Commentaires:

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 μL for immunoprecipitation. A HeLa whole cell lysate or mouse testis lysate are suitable for use as a positive control. Reactivity in other immunoassays is unknown. 

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur STAT4

Antigène: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Autre désignation: STAT4

Sujet:

Synonyms: rabbit anti-STAT4 Antibody, Signal transducer and activator of transcription 4, Stat4

Background: Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription.  STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members.  STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.

Gene Name: STAT4

ID gène: 20849

UniProt: P42228

Pathways: Signalistation JAK/STAT