CD86 anticorps (APC)

N° du produit ABIN6657892

N° du produit (Fournisseur): 200-526-n90

L’anticorps Rat Monoclonal anti-CD86 a été validé pour FACS, IHC et FM. Il convient pour détecter CD86 dans des échantillons de Souris.

Aperçu rapide pour CD86 anticorps (APC) (ABIN6657892)

Antigène: CD86

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD86 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: GL-1

Détail du produit anti-CD86 anticorps (APC)

N° du produit (Fournisseur): 200-526-n90

Fournisseur: Rockland

Fonction: CD86 Allophycocyanin Antibody

Réactivité croisée (Details): Cross reactivity with CD86 from other sources has not been tested.

Purification: Allophycocyanin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD86.

Stérilité: Sterile filtered

Immunogène: Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Commentaires:

Anti-CD86 is tested for FLOW and useful for Immunohistochemistry and Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Date de péremption: 6 months

Détails sur CD86

Antigène: CD86

Autre désignation: CD86

Sujet:

Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86, Cd86

Background: Anti-CD86 reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Gene Name: Cd86

ID gène: 12524

NCBI Accession: NP_062261

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation