SIX3 anticorps (Internal Region)

N° du produit ABIN6657910

N° du produit (Fournisseur): 200-201-a26

L’anticorps Cobaye Polyclonal anti-SIX3 a été validé pour WB, ELISA, IHC, IF, FM et DB. Il convient pour détecter SIX3 dans des échantillons de Souris. Il y a 5+ publications disponibles.

Aperçu rapide pour SIX3 anticorps (Internal Region) (ABIN6657910)

Antigène: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Reactivité: Souris

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp SIX3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Dot Blot (DB)

Détail du produit anti-SIX3 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 200-201-a26

Fournisseur: Rockland

Fonction: SIX3 Antibody

Réactivité croisée (Details): This antibody reacts with mouse Six3.

Purification: This product was purified by Protein A chromatography from monospecific antiserum.

Stérilité: Sterile filtered

Immunogène: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:5,000 - 1:25,000

Immunohistochemistry_Dilution: 1:250 - 1:500

IF_Microscopy_Dilution: 1:250 - 1:500

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Commentaires:

Suggested Applications: Multiplex
This Protein A purified antibody has been tested for use in Immunohistochemistry, immunofluorescence microscopy, dot blot, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-SIX3 (ABIN6657910)

Lipiec, Bem, Koziński, Chakraborty, Urban-Ciećko, Zajkowski, Dąbrowski, Szewczyk, Toval, Ferran, Nagalski, Wiśniewska: "TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." dans: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).

Grall, Gourain, Naïr, Martin, Birling, Freund, Duluc: "Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs." dans: Cell death & disease, Vol. 10, Issue 11, pp. 812, (2020) (PubMed).

OBrien, Guo, Bahrami-Samani, Park, Hasso, Lee, Fang, Kim, Guo, Hong, Peterson, Lozanoff, Raviram, Ren, Fogelgren, Smith, Valouev, McMahon: "Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies." dans: PLoS genetics, Vol. 14, Issue 1, pp. e1007181, (2018) (PubMed).

Madrigal, Moreno-Bravo, Martínez-López, Martínez, Puelles: "Mesencephalic origin of the rostral Substantia nigra pars reticulata." dans: Brain structure & function, Vol. 221, Issue 3, pp. 1403-12, (2016) (PubMed).

Moldrich, Gobius, Pollak, Zhang, Ren, Brown, Mori, De Juan Romero, Britanova, Tarabykin, Richards: "Molecular regulation of the developing commissural plate." dans: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3645-61, (2010) (PubMed).

Détails sur SIX3

Antigène: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Autre désignation: SIX3

Sujet:

Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: SIX3

ID gène: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus