SIX3 anticorps (Internal Region)

N° du produit ABIN6657913

N° du produit (Fournisseur): 600-401-a26s

L’anticorps Lapin Polyclonal anti-SIX3 a été validé pour WB, ELISA, IHC et FM. Il convient pour détecter SIX3 dans des échantillons de Souris. Il y a 6+ publications disponibles.

Aperçu rapide pour SIX3 anticorps (Internal Region) (ABIN6657913)

Antigène: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIX3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Détail du produit anti-SIX3 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-a26s

Fournisseur: Rockland

Fonction: Six3 Antibody

Réactivité croisée (Details): This antibody reacts with mouse Six3.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000-1:15,000

Immunohistochemistry_Dilution: 1:100-1:250

IF_Microscopy_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Commentaires:

Suggested Applications: IF, Multiplex
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 3 months

Références pour anticorps anti-SIX3 (ABIN6657913)

Brignani, Raj, Schmidt, Düdükcü, Adolfs, De Ruiter, Rybiczka-Tesulov, Verhagen, van der Meer, Broekhoven, Moreno-Bravo, Grossouw, Dumontier, Cloutier, Chédotal, Pasterkamp: "Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development." dans: Neuron, Vol. 107, Issue 4, pp. 684-702.e9, (2020) (PubMed).

Roy, Murphy, Deng, MacDonald, Bammler, Aldinger, Glass, Millen: "PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice." dans: eLife, Vol. 8, (2020) (PubMed).

Li, Gao, Wang, Song, Xu, Xie, Zhou, Pan, Peng, Zhang, Ge, Zhong: "Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations." dans: Frontiers in molecular neuroscience, Vol. 12, pp. 212, (2019) (PubMed).

Xu, Liang, Song, Zhang, Lindtner, Li, Wen, Liu, Guo, Qi, Wang, Wang, Li, You, Wang, Chen, Feng, Rubenstein, Yang: "SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression." dans: Development (Cambridge, England), Vol. 145, Issue 14, (2018) (PubMed).

Li, Xie, He, Zhou, Gao, Liu, Pan, Ge, Peng, Zhong: "Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." dans: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).

Diacou, Zhao, Zheng, Cvekl, Liu: "Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation." dans: Cell reports, Vol. 25, Issue 9, pp. 2510-2523.e4, (2018) (PubMed).

Détails sur SIX3

Antigène: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Autre désignation: Six3

Sujet:

Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: Six3

ID gène: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus