MIB1 anticorps (AA 465-732)

N° du produit ABIN6719398

Cet anticorps Lapin Polyclonal détecte spécifiquement MIB1 dans ELISA et WB. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour MIB1 anticorps (AA 465-732) (ABIN6719398)

Antigène: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MIB1 est non-conjugé

Application: ELISA, Western Blotting (WB)

Détail du produit anti-MIB1 anticorps

Épitope: AA 465-732

Fonction: Anti-Mib1/Mindbomb Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Mib1/Mindbomb Antibody Picoband® (ABIN6719398). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Barsi, J. C., Rajendra, R., Wu, J. I., Artzt, K. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech. Dev. 122: 1106-1117, 2005. 2. Henshall, D. C., Schindler, C. K., So, N. K., Lan, J.-Q., Meller, R., Simon, R. P.Death-associated protein kinase expression in human temporal lobe epilepsy. Ann. Neurol. 55: 485-494, 2004. 3. Jin, Y., Blue, E. K., Dixon, S., Shao, Z., Gallagher, P. J. A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK. J. Biol. Chem. 277: 46980-46986, 2002.

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MIB1

Antigène: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Autre désignation: MIB1

Sujet:

Synonyms: E3 ubiquitin-protein ligase MIB1, DAPK-interacting protein 1, DIP-1, Mind bomb homolog 1, RING-type E3 ubiquitin transferase MIB1 , Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2

Tissue Specificity: Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

Background: E3 ubiquitin-protein ligase MIB1 is an enzyme that in humans is encoded by the MIB1 gene. It is mapped to 18q11.2. This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).

Poids moléculaire: 110 kDa

ID gène: 57534

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2