ACLY anticorps (AA 1-180)

N° du produit ABIN6719406

Cet anticorps anti-ACLY est un anticorps Souris Monoclonal détectant ACLY dans WB, IHC, IF, FACS et ICC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour ACLY anticorps (AA 1-180) (ABIN6719406)

Antigène: ACLY (ATP Citrate Lyase (ACLY))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACLY est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 5I2

Détail du produit anti-ACLY anticorps

Épitope: AA 1-180

Fonction: Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ATP citrate lyase ACLY Antibody (monoclonal, 5I2) (ABIN6719406). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ACLY

Antigène: ACLY (ATP Citrate Lyase (ACLY))

Autre désignation: ACLY

Sujet:

Synonyms: ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Poids moléculaire: 121 kDa

ID gène: 47

UniProt: P53396

Pathways: L'effet Warburg