GLI2 anticorps (AA 721-1457)

N° du produit ABIN6719569

Cet anticorps Lapin Polyclonal détecte spécifiquement GLI2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour GLI2 anticorps (AA 721-1457) (ABIN6719569)

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-GLI2 anticorps

Épitope: AA 721-1457

Fonction: Anti-Zinc finger protein GLI2 GLI2 Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Zinc finger protein GLI2 GLI2 Antibody (ABIN6719569). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human GLI2 recombinant protein (Position: K721-D1457).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur GLI2

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Autre désignation: GLI2

Sujet:

Synonyms: Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein, GLI2, THP

Tissue Specificity: Expressed in breast cancers. Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines. Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines.

Background: GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Poids moléculaire: 210 kDa

ID gène: 2736

UniProt: P10070

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis