MEF2C anticorps

N° du produit ABIN6719606

L’anticorps Lapin Polyclonal anti-MEF2C a été validé pour WB, IHC et FACS. Il convient pour détecter MEF2C dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MEF2C anticorps (ABIN6719606)

Antigène: MEF2C (Myocyte Enhancer Factor 2C (MEF2C))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEF2C est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-MEF2C anticorps

Fonction: Anti-MEF2C Antibody Picoband®

Séquence: FHSPIGLTRP SPDERESPSV KRMRLSEGWA T

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MEF2C Antibody Picoband® (ABIN6719606). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2C, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Barbosa, A. C., Kim. M.-S., Ertunc, M., Adachi, M., Nelson, E. D., McAnally, J., Richardson, J. A., Kavalali, E. T., Monteggia, L. M., Bassel-Duby, R., Olson, E. N. MEF2C, a transcription factor that facilitates learning and memory by negative regulation of synapse numbers and function. Proc. Nat. Acad. Sci. 105: 9391-9396, 2008. 2. Chen, S. L., Dowhan, D. H., Hosking, B. M., Muscat, G. E. O. The steroid receptor coactivator, GRIP-1, is necessary for MEF-2C-dependent gene expression and skeletal muscle differentiation. Genes Dev. 14: 1209-1228, 2000. 3. Krainc, D., Haas, M., Ward, D. C., Lipton, S. A., Bruns, G., Leifer, D. Assignment of human myocyte-specific enhancer binding factor 2C (hMEF2C) to human chromosome 5q14 and evidence that MEF2C is evolutionarily conserved. Genomics 29: 809-811, 1995.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MEF2C

Antigène: MEF2C (Myocyte Enhancer Factor 2C (MEF2C))

Autre désignation: MEF2C

Sujet:

Synonyms: Myocyte-specific enhancer factor 2C , Myocyte enhancer factor 2C

Tissue Specificity: Expressed in brain and skeletal muscle.

Background: MEF2C (myocyte enhancer factor 2C), also called MADS box transcription enhancer factor 2, polypeptide C, is a protein that in humans is encoded by the MEF2C gene. MEF2C is a transcription factor in the Mef2 family. MEF2C, however, is induced late during myogenic differentiation and has a strict tissue-specific pattern of expression not seen in MEF2A or MEF2B. By fluorescence in situ hybridization, the human MEF2C is mapped to chromosome 5q14, a region with homology of synteny to the mouse location.MEF2C may be involved with maintenance of the differentiated state. Both MEF2A and Mef2c programmed similar profiles of gene expression in the heart that included genes involved in extracellular matrix remodeling, ion handling, and metabolism. NCOA2 mediates the coactivation of MEF2C-dependent transcription through interaction with the MADS box domain of MEF2C.

Poids moléculaire: 50-65 kDa

ID gène: 4208

UniProt: Q06413

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, BCR Signaling