PCNA anticorps (AA 185-261)

N° du produit ABIN672756

Cet anticorps anti-PCNA est un anticorps Lapin Polyclonal détectant PCNA dans WB, ELISA, FACS, IHC (p), IF (p), IF (cc) et IHC (fro). Adapté pour Humain, Souris, Rat, Poulet, Lapin et Mouton. Ce Primary Antibody a été cité dans 10+ publications.

Aperçu rapide pour PCNA anticorps (AA 185-261) (ABIN672756)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Poulet, Lapin, Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-PCNA anticorps

Épitope: AA 185-261

Réactivité croisée: Poulet, Humain, Souris, Lapin, Rat, Mouton

Homologie: Dog,Cow

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PCNA

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PCNA (ABIN672756)

Hu, Zuo, Li, Wang, Peng, Fang, Cui, Gao, Song, Zhou, Chen: "The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B 1." dans: Scientific reports, Vol. 8, Issue 1, pp. 1770, (2018) (PubMed).

He, Bian, Ma, Wang, Wang, Zhao: "Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2." dans: Oncology letters, Vol. 15, Issue 3, pp. 3977-3984, (2018) (PubMed).

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." dans: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, (2016) (PubMed).

Bi, Wang, Yang, Li, Liang, Han: "Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats." dans: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 851-856, (2015) (PubMed).

Li, Zhao, Wang, Chen, Lin, Zhu, Zheng, Wu, Cheng, Li, Bai, Fan, Liu: "Urotensin II promotes atherosclerosis in cholesterol-fed rabbits." dans: PLoS ONE, Vol. 9, Issue 4, pp. e95089, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." dans: Theriogenology, (2014) (PubMed).

Song, Yan, An, Hao, Guo, Zhang, Li, Li, Sun: "Potential contribution of SOCC to cerebral vasospasm after experimental subarachnoid hemorrhage in rats." dans: Brain research, Vol. 1517, pp. 93-103, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." dans: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet:

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy