Recombinant PIM2 anticorps (Sortase,His tag,AVI tag)

N° du produit ABIN6731036

Cet anticorps anti-PIM2 Monoclonal Souris conjugué à Sortase,His tag,AVI tag (ABIN6731036) détecte spécifiquement PIM2 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant PIM2 anticorps (Sortase,His tag,AVI tag) (ABIN6731036)

Antigène: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PIM2 est conjugé à/à la Sortase,His tag,AVI tag

Application: Western Blotting (WB)

Détail du produit anti-PIM2 anticorps (Sortase,His tag,AVI tag)

Fonction: AbFlex Pim2 antibody (rAb)

Marque: AbFlex®

Attributs du produit: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a recombinant protein corresponding to human PIM2 (pim-2 oncogene).

Isotype: IgG2a

Information d'application

Indications d'application: WB: 0.5 - 2 μg/mL Note: For optimal results, we recommend the addition of 0.1 % Tween to all blocking solutions to reduce background. Individual optimization may be required. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61159.

Commentaires:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur PIM2

Antigène: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Autre désignation: Pim2

Sujet: AbFlex Pim2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody recognizes all three isoforms of human Pim2. PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-kB kinase/NF-kB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.

Poids moléculaire: 37 kDa

NCBI Accession: NP_006866