HNF4A anticorps (N-Term)

N° du produit ABIN214775

L’anticorps Lapin Polyclonal anti-HNF4A a été validé pour WB, IHC et IHC (p). Il convient pour détecter HNF4A dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Singe, Cheval, Lapin, Cobaye, Mouton, Roussette (Chauve-souris), Hamster et Poulet.

Aperçu rapide pour HNF4A anticorps (N-Term) (ABIN214775)

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Singe, Cheval, Lapin, Cobaye, Mouton, Roussette (Chauve-souris), Hamster, Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HNF4A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HNF4A anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human HNF4A / HNF4, immunogenic peptide sequence is present in isoforms 1, 2, 3, and 4.

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human HNF4A (P41235, NP_000448). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard (100%), Catfish, Stickleback, Zebrafish (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur HNF4A

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Autre désignation: HNF4A / HNF4

Sujet: Name/Gene ID: HNF4A
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: HNF4A, Hepatic nuclear factor 4 alpha, HNF4, HNF4a8, NR2A21, TCF, TCF-14, TCF14, MODY1, Transcription factor 14, Transcription factor HNF-4, HNF-4-alpha, HNF4 alpha, HNF4a7, HNF4a9, HNF4alpha, HNF4alpha10/11/12, MODY, NR2A1

ID gène: 3172

NCBI Accession: NP_000448

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process