HMGN1 anticorps (AA 36-85)

N° du produit ABIN6741544

L’anticorps Lapin Polyclonal anti-HMGN1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter HMGN1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Cheval et Singe.

Aperçu rapide pour HMGN1 anticorps (AA 36-85) (ABIN6741544)

Antigène: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HMGN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HMGN1 anticorps

Épitope: AA 36-85

Marque: IHC-plus™

Specificité: Human HMGN1

Homologie: Percent identity by BLAST analysis: Human, Bovine (100%) Rabbit (92%) Rat (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa36-85 of human HMGN1 (P05114, NP_004956). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey, Bovine, Horse, Armadillo (100%), Gibbon, Rabbit (92%), Opossum (91%), Platypus (86%), Marmoset, Pig (85%), Rat (84%), Mouse (76%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur HMGN1

Antigène: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Autre désignation: HMGN1

Sujet: Name/Gene ID: HMGN1

Synonyms: HMGN1, HMG14

ID gène: 3150

NCBI Accession: NP_004956

UniProt: P05114

Pathways: Chromatin Binding