PODXL anticorps (AA 301-350)

N° du produit ABIN6741605

Cet anticorps Lapin Polyclonal détecte spécifiquement PODXL dans WB, IHC et IHC (p). Il présente une réactivité envers Humain et Singe.

Aperçu rapide pour PODXL anticorps (AA 301-350) (ABIN6741605)

Antigène: PODXL (Podocalyxin-Like (PODXL))

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PODXL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PODXL anticorps

Épitope: AA 301-350

Marque: IHC-plus™

Specificité: Human PODXL

Homologie: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa301-350 of human PODXL (A6NHX8, NP_001018121). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%), Gorilla (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur PODXL

Antigène: PODXL (Podocalyxin-Like (PODXL))

Autre désignation: PODXL / Podocalyxin

Sujet: Name/Gene ID: PODXL

Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

ID gène: 5420

NCBI Accession: NP_001018121

UniProt: O00592

Pathways: Tube Formation