MAS1 anticorps (AA 187-236)

N° du produit ABIN6741611

Cet anticorps Lapin Polyclonal détecte spécifiquement MAS1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Hamster et Singe.

Aperçu rapide pour MAS1 anticorps (AA 187-236) (ABIN6741611)

Antigène: MAS1 (MAS1 Oncogene (MAS1))

Reactivité: Humain, Souris, Rat, Hamster, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MAS1 anticorps

Épitope: AA 187-236

Marque: IHC-plus™

Specificité: Human MAS1

Homologie: Percent identity by BLAST analysis: Mouse (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa187-236 of human MAS1 (P04201, NP_002368). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Hamster, Dolphin (100%), Bovine (92%), Panda, Opossum, Guinea pig (85%), Galago (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur MAS1

Antigène: MAS1 (MAS1 Oncogene (MAS1))

Autre désignation: MAS1 / MAS

Sujet: Name/Gene ID: MAS1
Subfamily: Orphan-A
Family: GPCR

Synonyms: MAS1, Mas proto-oncogene, Proto-oncogene Mas, MAS, MAS1 oncogene

ID gène: 4142

NCBI Accession: NP_002368

UniProt: P04201

Pathways: ACE Inhibitor Pathway, Regulation of Carbohydrate Metabolic Process