IDH1 anticorps (AA 290-339)

N° du produit ABIN6741619

Cet anticorps Lapin Polyclonal détecte spécifiquement IDH1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Chien, Singe, Cheval, Cobaye, Boeuf (Vache), Lapin, Mouton, Porc, Hamster, Poulet et Xenopus laevis.

Aperçu rapide pour IDH1 anticorps (AA 290-339) (ABIN6741619)

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivité: Humain, Souris, Rat, Chien, Singe, Cheval, Cobaye, Boeuf (Vache), Lapin, Mouton, Porc, Hamster, Poulet, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IDH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-IDH1 anticorps

Épitope: AA 290-339

Marque: IHC-plus™

Specificité: Human IDH1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa290-339 of human IDH1 (Q8LFC0, NP_005887). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Beetle (100%), Panda, Armadillo, Rice, Arabidopsis (92%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur IDH1

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Autre désignation: IDH1 / IDH

Sujet: Name/Gene ID: IDH1

Synonyms: IDH1, IDCD, IDP, IDPC, PICD, NADP(+)-specific ICDH, IDH, Oxalosuccinate decarboxylase

ID gène: 3417

NCBI Accession: NP_005887

UniProt: O75874

Pathways: L'effet Warburg