NFX1 anticorps (C-Term)

N° du produit ABIN6741622

Cet anticorps Lapin Polyclonal détecte spécifiquement NFX1 dans WB, IHC (p) et IHC. Il présente une réactivité envers Humain, Souris, Rat, Chien, Lapin, Boeuf (Vache) et Singe.

Aperçu rapide pour NFX1 anticorps (C-Term) (ABIN6741622)

Antigène: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Reactivité: Humain, Souris, Rat, Chien, Lapin, Boeuf (Vache), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Détail du produit anti-NFX1 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human NFX1

Homologie: Percent identity by BLAST analysis: Human (100%) Rat, Dog, Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human NFX1 (Q12986, NP_002495). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Marmoset (100%), Gorilla, Galago, Pig, Guinea pig (92%), Rat, Dog, Bovine, Rabbit (85%), Mouse (76%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NFX1

Antigène: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Autre désignation: NFX1

Sujet: Name/Gene ID: NFX1

Synonyms: NFX1, NFX2

ID gène: 4799

NCBI Accession: NP_002495

UniProt: Q12986