IGFBP4 anticorps (AA 180-229)

N° du produit ABIN6744434

Cet anticorps anti-IGFBP4 est un anticorps Lapin Polyclonal détectant IGFBP4 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chévre, Chien, Cheval, Lapin, Mouton et Roussette (Chauve-souris).

Aperçu rapide pour IGFBP4 anticorps (AA 180-229) (ABIN6744434)

Antigène: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chévre, Chien, Cheval, Lapin, Mouton, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IGFBP4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-IGFBP4 anticorps

Épitope: AA 180-229

Marque: IHC-plus™

Specificité: Human IGFBP4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Sheep, Dog, Bovine, Horse (100%) Pig, Guinea pig, Chicken (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa180-229 of human IGFBP4 (P22692, NP_001543). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse (100%), Marmoset, Hamster, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur IGFBP4

Antigène: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Autre désignation: IGFBP4 / IGFBP-4

Sujet: Name/Gene ID: IGFBP4

Synonyms: IGFBP4, BP-4, HT29-IGFBP, IGF-binding protein 4, IBP-4, IGFBP-4, IBP4

ID gène: 3487

NCBI Accession: NP_001543

UniProt: P22692

Pathways: Signalisation WNT, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process