MKNK2 anticorps (AA 36-85)
Aperçu rapide pour MKNK2 anticorps (AA 36-85) (ABIN6744440)
Antigène
Voir toutes MKNK2 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 36-85
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Marque
- IHC-plus™
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Specificité
- Human MKNK2 / MNK2
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Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig, Zebrafish (100%) Bovine (92%) Xenopus (83%).
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Purification
- Immunoaffinity purified
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Immunogène
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Synthetic peptide located between aa36-85 of human MKNK2 (Q9HBH9, NP_951009). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Zebra finch, Stickleback, Zebrafish (100%), Bovine (92%), Xenopus (91%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Indications d'application
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Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))
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Autre désignation
- MKNK2 / MNK2
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Sujet
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Name/Gene ID: MKNK2
Subfamily: MNK
Family: Protein Kinase
Synonyms: MKNK2, GPRK7, MNK2, MAPK-Interacting Kinase 2 -
ID gène
- 2872
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NCBI Accession
- NP_951009
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UniProt
- Q9HBH9
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Pathways
- Signalisation MAPK
Antigène
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