Hexokinase 2 anticorps (AA 74-123)

N° du produit ABIN462257

L’anticorps Lapin Polyclonal anti-Hexokinase 2 a été validé pour WB, IHC et IHC (p). Il convient pour détecter Hexokinase 2 dans des échantillons de Humain, Souris, Rat, Chien, Lapin, Porc, Boeuf (Vache), Cheval, Singe, Cobaye, Poisson zèbre (Danio rerio), Hamster et Roussette (Chauve-souris).

Aperçu rapide pour Hexokinase 2 anticorps (AA 74-123) (ABIN462257)

Antigène: Hexokinase 2 (HK2)

Reactivité: Humain, Souris, Rat, Chien, Lapin, Porc, Boeuf (Vache), Cheval, Singe, Cobaye, Poisson zèbre (Danio rerio), Hamster, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Hexokinase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Hexokinase 2 anticorps

Épitope: AA 74-123

Marque: IHC-plus™

Specificité: Human HK2 / Hexokinase 2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Chicken, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sablefish, Stickleback (92%), Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Hexokinase 2

Antigène: Hexokinase 2 (HK2)

Autre désignation: HK2 / Hexokinase 2

Sujet: Name/Gene ID: HK2
Subfamily: Hexokinase
Family: Non-protein Kinase

Synonyms: HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2

ID gène: 3099

NCBI Accession: NP_000180

UniProt: P52789

Pathways: Signalisation PI3K-Akt, Carbohydrate Homeostasis, L'effet Warburg