LDLR anticorps (AA 184-196)

N° du produit ABIN233315

L’anticorps Lapin Polyclonal anti-LDLR a été validé pour WB, IHC, ELISA, FACS et IHC (fro). Il convient pour détecter LDLR dans des échantillons de Humain et Rat.

Aperçu rapide pour LDLR anticorps (AA 184-196) (ABIN233315)

Antigène: LDLR (Low Density Lipoprotein Receptor (LDLR))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LDLR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-LDLR anticorps

Épitope: AA 184-196

Specificité: Recognizes human LDLR. Does not inhibit binding of LDL. In Western Blot, recognizes the 160kD of LDLR and a 120kD band of the LDLR precursor from fibroblasts, hepatocytes and monocytic cells cultured in the presence of lipoprotein-deficient serum. Sequence not conserved in VLVL Receptor and LRP. Species cross-reactivity: rat. Species sequence Homology: mouse and bovine.

Purification: Protein A purified

Immunogène: Synthetic peptide of the complement homology region within the ligand binding site of the LDL receptor extracellular domain. Epitope between aa184-196, the linker region between repeats 4 and 5.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, Flo (1:5), IHC, IHC-Fr (1:10), WB (1:50 - 1:100)

Usage: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Western Blot: 1:50-1:100 using ECL (non-reducing). FACS: 1:5. Immunohistochemistry: 1:10. Note: It is best to work with partially purified material. In the case of rat (probably also mouse), induce LDLR expression prior to the isolation of culture cells or tissue homogenate. It is always better to use cultured cells. In rat, use estradiol to induce LDLR expression. Positive control: HepG2 cells and liver sections.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.5 % BSA.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur LDLR

Antigène: LDLR (Low Density Lipoprotein Receptor (LDLR))

Autre désignation: LDLR / LDL Receptor

Sujet: Name/Gene ID: LDLR

Synonyms: LDLR, FHC, LDL receptor, LDLCQ2

ID gène: 3949

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism