CDK1 anticorps (pTyr15) (HRP)

N° du produit ABIN684022

Détail du produit anti-CDK1 anticorps (HRP)

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Épitope: pTyr15

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDK1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This phosphorylation site is homologous in the listed cross reactive species at the specified location.

Réactivité croisée: Humain, Souris

Homologie: Rat,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human CDK1 around the phosphorylation site of Tyr15 [GT(p-Y)GV]

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CDK1

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: CDK1 (CDK1 Produits)

Synonymes: anticorps CDC2, anticorps CDC28A, anticorps P34CDC2, anticorps Cdc2, anticorps Cdc2a, anticorps p34, anticorps cdc2, anticorps wu:fc30e01, anticorps zgc:92032, anticorps PSTAIR, anticorps cdc-2, anticorps cdc2-a, anticorps cdc28a, anticorps cdc2x1.1, anticorps p34cdc2, anticorps xcdc2, anticorps 5363, anticorps CDCDm, anticorps CDK1, anticorps CDK1/CDC2, anticorps CG5363, anticorps Cdk-1, anticorps Cdk1, anticorps Dcdc2, anticorps Dm cdc2, anticorps DmCdc2, anticorps DmCdk1, anticorps Dmcdc2, anticorps Dmel\\CG5363, anticorps cdc, anticorps cdc2Dm, anticorps cdk1, anticorps dCdk1, anticorps group 4, anticorps l(2)31Eh, anticorps CDC2A, anticorps CDC2AAT, anticorps CDK2, anticorps CDKA1, anticorps CDKA;1, anticorps CYCLIN-DEPENDENT KINASE A;1, anticorps cell division control 2, anticorps cdc2-b, anticorps cdc2a, anticorps cdc2x1.2, anticorps POL3, anticorps pold, anticorps cyclin dependent kinase 1, anticorps cyclin dependent kinase like 1, anticorps Cell division control protein 2 1, anticorps cyclin-dependent kinase 1, anticorps cyclin-dependent kinase 1 S homeolog, anticorps Cyclin-dependent kinase 1, anticorps cell division control 2, anticorps cell division control protein2 homolog, anticorps cyclin-dependent kinase 1 L homeolog, anticorps polymerase (DNA directed), delta 1, catalytic subunit L homeolog, anticorps cyclin-dependent protein kinase Cdk1/Cdc2, anticorps CDK1, anticorps CDKL1, anticorps POPTR_0004s14080g, anticorps Cdk1, anticorps cdk1, anticorps cdk1.S, anticorps CDC2, anticorps cdc2, anticorps cdk-1, anticorps cdk1.L, anticorps pold1.L

Sujet:

Synonyms: CDC2, CDC28A, P34CDC2, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDKN1

Background: Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset, promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair.

ID gène: 983

UniProt: P06493

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA