CDK9 anticorps (pThr186)

N° du produit ABIN684088

Détail du produit anti-CDK9 anticorps

Antigène: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Épitope: pThr186

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDK9 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human CDK9 around the phosphorylation site of Thr186

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CDK9

Antigène: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Autre désignation: CDK9 (CDK9 Produits)

Synonymes: anticorps C-2k, anticorps CDC2L4, anticorps CTK1, anticorps PITALRE, anticorps TAK, anticorps wu:fb22e09, anticorps wu:fc06c02, anticorps c-2k, anticorps cdc2l4, anticorps cdk9, anticorps ctk1, anticorps pitalre, anticorps tak, anticorps CDK9, anticorps CG5179, anticorps Dmel\\CG5179, anticorps P-TEF, anticorps P-TEFb, anticorps PTefb, anticorps cyclin dependent kinase 9, anticorps cyclin-dependent kinase 9 (CDC2-related kinase), anticorps cyclin-dependent kinase 9 L homeolog, anticorps cyclin-dependent kinase 9, anticorps Cyclin-dependent kinase 9, anticorps cyclin-dependent kinase 9 S homeolog, anticorps CDK9, anticorps cdk9, anticorps cdk9.L, anticorps Cdk9, anticorps cdk9.S

Sujet:

Synonyms: TAK, C-2k, CTK1, CDC2L4, PITALRE, Cyclin-dependent kinase 9, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit, CDK9

Background: Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3, integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein, the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement.

ID gène: 1025

UniProt: P50750

Pathways: Cycle Cellulaire