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KRT77, KRT76 anticorps (PE)

Reactivité: Humain, Rat, Singe, Souris, Lapin, Poulet, Boeuf (Vache), Chien FACS, IF, T Hôte: Souris Monoclonal SPM115-SPM116 PE
N° du produit ABIN6933732
  • Antigène
    KRT77, KRT76
    Reactivité
    Humain, Rat, Singe, Souris, Lapin, Poulet, Boeuf (Vache), Chien
    Hôte
    • 5
    • 3
    Souris
    Clonalité
    • 7
    • 1
    Monoclonal
    Conjugué
    • 7
    • 1
    PE
    Application
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)
    Specificité
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This MAb is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
    Purification
    Purified by Protein A/G
    Immunogène
    Human epidermal keratin
    Clone
    SPM115-SPM116
    Isotype
    IgG1 kappa
  • Indications d'application

    Positive Control: Skin, Adeno- or Squamous carcinomas.

    Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    100 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA and 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    KRT77, KRT76
    Autre désignation
    KRT77 & KRT76
    Poids moléculaire
    67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
    ID gène
    374454, 51350
    UniProt
    Q7Z794, Q01546
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