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KRT77, KRT76 anticorps

Reactivité: Humain FACS, IF, WB, IHC (f) Hôte: Souris Monoclonal SPM115-SPM116 unconjugated
N° du produit ABIN6937743
  • Antigène
    KRT77, KRT76
    Reactivité
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Humain
    Hôte
    • 5
    • 3
    Souris
    Clonalité
    • 7
    • 1
    Monoclonal
    Conjugué
    • 6
    • 1
    • 1
    Inconjugué
    Application
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificité
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
    Réactivité croisée (Details)
    Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogène
    Human epidermal keratin
    Clone
    SPM115-SPM116
    Isotype
    IgG1 kappa
  • Indications d'application

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 2 hours at RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Skin, Adeno- or Squamous carcinomas.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Agent conservateur
    Azide free
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Date de péremption
    24 months
  • Antigène
    KRT77, KRT76
    Autre désignation
    KRT77 & KRT76
    Sujet
    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, pan (Epithelial Marker)
    Cellular localisation: Cytoplasmic
    Poids moléculaire
    67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
    ID gène
    374454, 51350, 334989
    UniProt
    Q7Z794, Q01546
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