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KRT77, KRT76 anticorps

Reactivité: Humain, Rat IHC, StM Hôte: Souris Monoclonal KRTL-1077-KRTH-1076 unconjugated
N° du produit ABIN6941307
  • Antigène
    KRT77, KRT76
    Reactivité
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Humain, Rat
    Hôte
    • 5
    • 3
    Souris
    Clonalité
    • 7
    • 1
    Monoclonal
    Conjugué
    • 6
    • 1
    • 1
    Inconjugué
    Application
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    Immunohistochemistry (IHC), Staining Methods (StM)
    Specificité
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
    Réactivité croisée (Details)
    Shows broad species reactivity.
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant human KRT77 and KRT76 protein
    Clone
    KRTL-1077-KRTH-1076
    Isotype
    IgG1 kappa
  • Indications d'application

    Positive Control: Skin, Adeno- or Squamous carcinomas.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    KRT77, KRT76
    Autre désignation
    KRT77 & KRT76
    Poids moléculaire
    67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
    ID gène
    374454, 51350
    UniProt
    Q7Z794, Q01546
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