Cyclin D1 anticorps (PE)

N° du produit ABIN6934200

L’anticorps Souris Monoclonal anti-Cyclin D1 a été validé pour FACS, IF et T. Il convient pour détecter Cyclin D1 dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour Cyclin D1 anticorps (PE) (ABIN6934200)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: DCS-6

Détail du produit anti-Cyclin D1 anticorps (PE)

Specificité: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Purification: Purified by Protein A/G

Immunogène: Human recombinant full length cyclin D1 protein

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60 % of mantle cell lymphomas and ~40 % of breast carcinomas are positive.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: CCND1

Poids moléculaire: 36kDa

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling