Glypican 3 anticorps (AA 511-580) (CF®555)

N° du produit ABIN6934330

Cet anticorps anti-Glypican 3 est un anticorps Souris Monoclonal détectant Glypican 3 dans FACS, IF, IHC et StM. Adapté pour Humain.

Aperçu rapide pour Glypican 3 anticorps (AA 511-580) (CF®555) (ABIN6934330)

Antigène: Glypican 3 (GPC3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Glypican 3 est conjugé à/à la CF®555

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: SPM595

Détail du produit anti-Glypican 3 anticorps (CF®555)

Épitope: AA 511-580

Purification: Purified by Protein A/G

Immunogène: A recombinant fragment containing amino acids 511-580 of human glypican-3

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: 293T cells. Hepatocellular carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Glypican 3

Antigène: Glypican 3 (GPC3)

Autre désignation: GPC3

Sujet: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Poids moléculaire: 67kDa

ID gène: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process