CD63 anticorps (CF®488A)

N° du produit ABIN6935954

Cet anticorps Souris Monoclonal détecte spécifiquement CD63 dans FACS, IF et T. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour CD63 anticorps (CF®488A) (ABIN6935954)

Antigène: CD63

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est conjugé à/à la CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: NKI-C3

Détail du produit anti-CD63 anticorps (CF®488A)

Specificité: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Purification: Purified by Protein A/G

Immunogène: Smooth plasma membrane fraction of MeWo cells

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD63

Antigène: CD63

Autre désignation: CD63

Poids moléculaire: 26kDa (core protein), 30-60kDa (glycosylated)

ID gène: 967

UniProt: P08962