ACTN2 anticorps (AA 557-692)

N° du produit ABIN6936359

Cet anticorps Souris Monoclonal détecte spécifiquement ACTN2 dans WB et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour ACTN2 anticorps (AA 557-692) (ABIN6936359)

Antigène: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACTN2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ACTN2-3292

Détail du produit anti-ACTN2 anticorps

Épitope: AA 557-692

Specificité: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skeletal or Cardiac Muscle.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ACTN2

Antigène: ACTN2 (Actinin, alpha 2 (ACTN2))

Autre désignation: ACTN2

Sujet: Actin binding protein, Alpha actinin skeletal muscle, isoform 2, CMD1AA, F-actin cross-linking protein,Sarcomeric Actinin Alpha 2 / ACTN2
Cellular localisation: Cytoplasmic

Poids moléculaire: 103kDa

ID gène: 88, 498178

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity