Recombinant Aurora Kinase B anticorps (AA 89-251)

N° du produit ABIN6936445

Cet anticorps anti-Aurora Kinase B Monoclonal Lapin (Clone AURKB-3121R) (ABIN6936445) détecte spécifiquement Aurora Kinase B dans IHC et WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant Aurora Kinase B anticorps (AA 89-251) (ABIN6936445)

Antigène: Aurora Kinase B (AURKB)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Aurora Kinase B est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: AURKB-3121R

Détail du produit anti-Aurora Kinase B anticorps

Épitope: AA 89-251

Fonction: Recombinant Aurora B (Proliferation Marker) Antibody

Specificité: Recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Tonsil, Placenta, Hodgkin's Lymphoma or Colon Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Aurora Kinase B

Antigène: Aurora Kinase B (AURKB)

Autre désignation: AURKB

Sujet:

AIK2; AIM-1; ARK-2; AurB; AURKB; Aurora-1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; IPL1; Protein phosphatase 1 regulatory subunit 48 (PPP1R48); Serine/threonine-protein kinase 12; Serine/threonine-protein kinase aurora-B; STK1; STK12; STK5

HeLa cells. Tonsil, Hodgkin's Lymphoma or Colon Carcinoma., Placenta, Human Liver

Poids moléculaire: 39kDa

ID gène: 9212, 442658

UniProt: Q96GD4

Pathways: TCR Signaling, Cycle Cellulaire, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades