BRCA1 anticorps (AA 445-620)

N° du produit ABIN6936512

Cet anticorps Souris Monoclonal détecte spécifiquement BRCA1 dans IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour BRCA1 anticorps (AA 445-620) (ABIN6936512)

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: BRCA1-1398

Détail du produit anti-BRCA1 anticorps

Épitope: AA 445-620

Specificité: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human BRCA1 protein fragment (around aa445-620) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (2-5 μg/mL for 60 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 or HeLa cells. Breast, Ovarian or Renal Cell Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1

Sujet: BRCA1, Breast and ovarian cancer susceptibility protein 1, Breast Cancer 1 Early Onset, Breast cancer type 1 susceptibility protein, BROVCA1, IRIS, PNCA4, PPP1R53, Protein phosphatase 1 regulatory subunit 53, PSCP, RING finger protein 53, RNF53,BRCA1 (Breast Marker)
Cellular localisation: Cytoplasmic and Nuclear

Poids moléculaire: 220kDa

ID gène: 672, 194143

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus