Cyclin A1 anticorps

N° du produit ABIN6936580

Cet anticorps anti-Cyclin A1 est un anticorps Souris Monoclonal détectant Cyclin A1 dans WB. Adapté pour Xenopus laevis.

Aperçu rapide pour Cyclin A1 anticorps (ABIN6936580)

Antigène: Cyclin A1 (CCNA1)

Reactivité: Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A1 est non-conjugé

Application: Western Blotting (WB)

Clone: XLA1-3

Détail du produit anti-Cyclin A1 anticorps

Specificité: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1.

Réactivité croisée (Details): Xenopus laevis

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length Xenopus laevis Cyclin A1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cyclin A1

Antigène: Cyclin A1 (CCNA1)

Autre désignation: ccna1

Sujet: CCN1, CCNA, CCNA2, Cyclin A1,Cyclin A1
Cellular localisation: Nucleus.

Poids moléculaire: 65kDa

ID gène: 4267

UniProt: P18606

Pathways: Apoptose, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA