Cyclin A anticorps
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- Antigène Voir toutes Cyclin A (CCNA2) Anticorps
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
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Reactivité
- Boeuf (Vache)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Cyclin A est non-conjugé
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
- Réactivité croisée (Details)
- Human. Mouse. Cow. African Green Monkey. Mink (Mustela).
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Full length bovine Cyclin A protein
- Clone
- E67
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CCNA2 Anticorps primaire
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K-562 and HeLa cells. Human tonsil or lymph node.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
- Autre désignation
- CCNA2 (CCNA2 Produits)
- Synonymes
- anticorps CCN1, anticorps CCNA, anticorps AA408589, anticorps Ccn-1, anticorps Ccn1, anticorps Ccna, anticorps CycA2, anticorps Cyca, anticorps cb671, anticorps chunp6924, anticorps wu:fi36f03, anticorps CYCA, anticorps Cyclin-A2, anticorps ccna2, anticorps ccn1, anticorps ccna, anticorps ccna1, anticorps Cyclin-A, anticorps cyclinA, anticorps cyclin A2, anticorps cyclin A2 S homeolog, anticorps cyclin A2 L homeolog, anticorps CCNA2, anticorps Ccna2, anticorps ccna2, anticorps ccna2.S, anticorps ccna2.L
- Sujet
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CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2,Cyclin A2 (S- & G2-phase Cyclin)
Cellular localisation: Predominantly nuclear - Poids moléculaire
- 54kDa
- ID gène
- 890, 58974
- UniProt
- P20248
- Pathways
- Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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