Cyclin A anticorps

N° du produit ABIN6936581

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin A dans IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Boeuf (Vache), Green Monkey et Vison.

Aperçu rapide pour Cyclin A anticorps (ABIN6936581)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivité: Humain, Souris, Boeuf (Vache), Green Monkey, Vison

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: E67

Détail du produit anti-Cyclin A anticorps

Fonction: Cyclin A2 (S- & G2-phase Cyclin) Antibody

Specificité: It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Réactivité croisée (Details): Human. Mouse. Cow. African Green Monkey. Mink (Mustela).

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Full length bovine Cyclin A protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562 and HeLa cells. Human tonsil or lymph node.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: CCNA2

Sujet:

CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

K-562 and HeLa cells. Human tonsil or lymph node.

Poids moléculaire: 54kDa

ID gène: 890, 58974

UniProt: P20248

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA