Recombinant CD44 anticorps

N° du produit ABIN6936715

Cet anticorps anti-CD44 est un anticorps Souris Monoclonal détectant CD44 dans FACS, WB, IHC, IF, Func et IHC (f). Adapté pour Humain.

Aperçu rapide pour Recombinant CD44 anticorps (ABIN6936715)

Antigène: CD44

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD44 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RHCAM-918

Détail du produit anti-CD44 anticorps

Specificité: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Réactivité croisée (Details): Human, Baboon, and Green Monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human CD44 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells (FACS). HeLa cell lysate (WB). Paracortex in tonsil or lymph node (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD44

Antigène: CD44

Autre désignation: CD44

Sujet: LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group,CD44 / HCAM Std. (Cancer Stem Cell Marker)
Cellular localisation: Cell Surface

Poids moléculaire: 80-95kDa

ID gène: 960, 502328

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling