CD47 anticorps (AA 18-135)

N° du produit ABIN6936741

L’anticorps Souris Monoclonal anti-CD47 a été validé pour FACS, WB, IHC, IF, ELISA, IHC (f) et Coat. Il convient pour détecter CD47 dans des échantillons de Humain.

Aperçu rapide pour CD47 anticorps (AA 18-135) (ABIN6936741)

Antigène: CD47

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: CD47-2937

Détail du produit anti-CD47 anticorps

Épitope: AA 18-135

Specificité: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

Isotype: IgG2c kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD47

Antigène: CD47

Autre désignation: CD47

Sujet: Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen,CD47 / IAP (Integrin Associated Protein)
Cellular localisation: Cell Surface

Poids moléculaire: ~50kDa

ID gène: 961, 446414

UniProt: Q08722