CD63 anticorps (AA 100-197)

N° du produit ABIN6936772

Détail du produit anti-CD63 anticorps

Antigène: CD63

Épitope: AA 100-197

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndromeand is strongly expressed during the early stages of melanoma progression.

Réactivité croisée (Details): Human. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment (around aa 100-197) of humanCD63 protein

Clone: LAMP3-2790

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Spleen,melanoma or lymphoma tissues (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD63

Antigène: CD63

Autre désignation: CD63 (CD63 Produits)

Synonymes: anticorps C75951, anticorps ME491, anticorps Tspan30, anticorps LAMP-3, anticorps MLA1, anticorps OMA81H, anticorps TSPAN30, anticorps TSP29FA, anticorps CD63 antigen, anticorps CD63 molecule, anticorps Cd63 molecule, anticorps Cd63, anticorps CD63

Sujet: Gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
Cellular localisation: Cell surface and cytoplasmic

Poids moléculaire: 26kDa (core protein), 30-60kDa (glycosylated)

ID gène: 967, 445570

UniProt: P08962