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CEACAM1/5 anticorps

CEACAM1/5 Reactivité: Humain FACS, WB, IHC (f), IF Hôte: Souris Monoclonal COL-1 unconjugated
N° du produit ABIN6936928
  • Antigène Tous les produits CEACAM1/5
    CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))
    Reactivité
    Humain
    Hôte
    • 7
    • 5
    Souris
    Clonalité
    • 10
    • 2
    Monoclonal
    Conjugué
    • 11
    • 1
    Cet anticorp CEACAM1/5 est non-conjugé
    Application
    • 6
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
    Specificité
    This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
    Réactivité croisée (Details)
    Human.
    Purification
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogène
    Human colon carcinoma extract
    Clone
    COL-1
    Isotype
    IgG2a kappa
  • Indications d'application

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF7 or 293T cells. Human colon carcinoma.

    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Agent conservateur
    Azide free
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Date de péremption
    24 months
  • Antigène
    CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))
    Autre désignation
    CEACAM1 & CEACAM5 (CEACAM1/5 Produits)
    Synonymes
    anticorps CD66e, anticorps CEA, anticorps carcinoembryonic antigen related cell adhesion molecule 5, anticorps CEACAM5
    Sujet
    Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),Carcinoembryonic Antigen (CEA) / CD66
    Cellular localisation: Cytoplasmic and luminal surface
    Poids moléculaire
    80-200kDa
    ID gène
    1048, 709196
    UniProt
    P06731
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