Recombinant Cytochrome C anticorps (AA 1-80)

N° du produit ABIN6937068

L’anticorps Souris Monoclonal anti-Cytochrome C a été validé pour WB, FACS et IHC (f). Il convient pour détecter Cytochrome C dans des échantillons de Pigeon.

Aperçu rapide pour Recombinant Cytochrome C anticorps (AA 1-80) (ABIN6937068)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Type d'anticorp: Recombinant Antibody

Reactivité: Pigeon

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RCYCS-1010

Détail du produit anti-Cytochrome C anticorps

Épitope: AA 1-80

Specificité: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Réactivité croisée (Details): Human. Mouse. Rat. Horse. Dog. Pigeon. Frog. Drosophila.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or cardiac muscle. Heart tissue lysate.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: CYCS

Sujet: CYC, CYCS, HCS, THC4,Cytochrome C (Mitochondrial Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 15kDa

ID gène: 54205, 437060

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity