Recombinant Cytochrome C anticorps

N° du produit ABIN6937069

Détail du produit anti-Cytochrome C anticorps

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

Réactivité croisée (Details): Human, Mouse, Rat, Horse, Dog, Pigeon, Frog and Drosophila. Others-not known

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human CYCS protein

Clone: CYCS-3128R

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: CYCS (CYCS Produits)

Synonymes: anticorps CYC, anticorps HCS, anticorps THC4, anticorps cyct, anticorps cyc, anticorps hcs, anticorps CYCS, anticorps cytc, anticorps CYCSA, anticorps CG17903, anticorps Cyt C, anticorps Cyt c, anticorps Cyt-c2, anticorps CytC-2, anticorps Cytc, anticorps Cytc-p, anticorps DC4, anticorps DMc01, anticorps Dmel\\CG17903, anticorps cyt c, anticorps cyt c-p, anticorps cyt-c-p, anticorps cyt-c2, anticorps cyt.c, anticorps cytochrome c, anticorps dc4, anticorps pDMc01, anticorps wu:fk52b01, anticorps zgc:86706, anticorps ENSMUSG00000058927, anticorps cytochrome c, somatic, anticorps cytochrome c, somatic S homeolog, anticorps Cytochrome c proximal, anticorps cytochrome c, somatic b, anticorps cytochrome c, somatic pseudogene, anticorps cytochrome c-like, anticorps cytochrome c, anticorps CYCS, anticorps cycs.S, anticorps cycs, anticorps Cycs, anticorps Cyt-c-p, anticorps cycsb, anticorps Gm10053, anticorps LOC100724240, anticorps CYC, anticorps LOC100847700, anticorps LOC101107954, anticorps LOC106829744, anticorps LOC106992390, anticorps LOC102085712

Sujet: CYC, CYCS, HCS, THC4,Cytochrome C (Mitochondrial Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 15kDa

ID gène: 54205, 437060

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity