GRP94 anticorps

N° du produit ABIN6937437

L’anticorps anti-GRP94 Monoclonal Rat est utilisé pour la détection de GRP94 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval et Xenopus laevis. Il a été validé pour WB, IHC, FACS et IF.

Aperçu rapide pour GRP94 anticorps (ABIN6937437)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 9G10-F8-2

Détail du produit anti-GRP94 anticorps

Fonction: GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody

Specificité: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Réactivité croisée (Details): Human. Monkey. Horse. Cow. Sheep. Pig. Rabbit. Dog. Rat. Mouse. Hamster. Guinea Pig. Chicken. Xenopus laevis.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Purified glucose regulated protein 94 (grp94) from chicken oviducts

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, U20S cells. About 50 % of breast tumors express grp94 protein.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: HSP90B1

Sujet:

Glucose Regulated Protein 94, 94 kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90 kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

HePG2, U20S cells. About 50 % of breast tumors express grp94 protein.

Poids moléculaire: 94kDa

ID gène: 7184, 192374

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades