PCNA anticorps
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- Antigène Voir toutes PCNA Anticorps
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp PCNA est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
- Réactivité croisée (Details)
- Human. Dog. Shows broad species reactivity.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant full length human PCNA protein
- Clone
- PCNA-694
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product PCNA Anticorps primaire
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Tonsil or reactive lymph node.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Autre désignation
- PCNA (PCNA Produits)
- Synonymes
- anticorps PCNAR, anticorps Pcna/cyclin, anticorps pcna, anticorps AO090003000846, anticorps 53/13, anticorps CG9193, anticorps DmPCNA, anticorps Dmel\\CG9193, anticorps MUS209, anticorps Mus209, anticorps PCNA, anticorps PCNA/mus209, anticorps Pcna, anticorps dPCNA, anticorps l(2)02448, anticorps l(2)56F, anticorps l(2)56Fa, anticorps l(2)s1534, anticorps cb16, anticorps fb36g03, anticorps wu:fa28e03, anticorps wu:fb36g03, anticorps pcna-A, anticorps proliferating cell nuclear antigen, anticorps DNA polymerase III sliding clamp, anticorps Proliferating cell nuclear antigen, anticorps Proliferating cell nuclear antigen-like, anticorps proliferating cell nuclear antigen L homeolog, anticorps proliferating cell nuclear antigen 1, anticorps PCNA, anticorps Pcna, anticorps pcna, anticorps MMP_RS08810, anticorps ANI_1_512014, anticorps AOR_1_1500154, anticorps MGC53867, anticorps pcna.L, anticorps pcna1, anticorps LOC106420145, anticorps pcn1
- Sujet
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Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein,PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)
Cellular localisation: Predominantly nuclear, some cytoplasmic - Poids moléculaire
- 36kDa
- ID gène
- 5111, 147433, 728886
- UniProt
- P12004
- Pathways
- Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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