PCNA anticorps

N° du produit ABIN6938132

Cet anticorps Souris Monoclonal détecte spécifiquement PCNA dans WB et IHC (f). Il présente une réactivité envers Rat.

Aperçu rapide pour PCNA anticorps (ABIN6938132)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PC5

Détail du produit anti-PCNA anticorps

Specificité: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Réactivité croisée (Details): Human. Mouse. Rat. Yeast. Dog

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Rat PCNA/protein A fusion protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein,PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)
Cellular localisation: Predominantly nuclear, some cytoplasmic

Poids moléculaire: 36kDa

ID gène: 5111, 147433, 728886

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy