Prohibitin anticorps (AA 167-261)
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- Antigène Voir toutes Prohibitin (PHB) Anticorps
- Prohibitin (PHB)
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Épitope
- AA 167-261
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Prohibitin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)
- Clone
- PHB-1881
- Isotype
- IgG2c kappa
- Top Product
- Discover our top product PHB Anticorps primaire
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- Indications d'application
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, A431 or MCF-7, Ramos, Jurkat, K562 cells. Liver, Kidney, Heart or Endometrium.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- Prohibitin (PHB)
- Autre désignation
- PHB (PHB Produits)
- Synonymes
- anticorps phb1, anticorps MGC75944, anticorps PHB, anticorps Phb, anticorps ACYPI000080, anticorps phb, anticorps PHB1, anticorps Bap32, anticorps sr:nyz128, anticorps wu:fa18f07, anticorps wu:fb09f05, anticorps prohibitin1, anticorps xphb1, anticorps prohibitin, anticorps prohibitin-2, anticorps prohibitin L homeolog, anticorps PHB, anticorps phb, anticorps LOC484431, anticorps LOC5576054, anticorps Phb, anticorps phb.L
- Sujet
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Epididymis luminal protein 215, Epididymis secretory sperm binding protein Li 54e, HEL 215, HEL S 54e, PHB, PHB1, Prohibitin,Prohibitin (Mitochondrial Marker)
Cellular localisation: Cytoplasmic (Mitochondria) - Poids moléculaire
- 30kDa
- ID gène
- 5245, 514303
- UniProt
- P35232
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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