Melanoma gp100 anticorps (AA 376-502)

N° du produit ABIN6938385

L’anticorps Souris Monoclonal anti-Melanoma gp100 a été validé pour WB, ELISA, IHC (f) et Coat. Il convient pour détecter Melanoma gp100 dans des échantillons de Humain.

Aperçu rapide pour Melanoma gp100 anticorps (AA 376-502) (ABIN6938385)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: PMEL-2037

Détail du produit anti-Melanoma gp100 anticorps

Épitope: AA 376-502

Specificité: The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26 kDa and 34-38 kDa fragments. Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: A recombinant fragment (around aa 376-502) of human SILV protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: CACO-38 or SK-MEL-28 cells. Melanoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: SILV

Sujet: 95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, Premelanosome protein (PMEL17), Secreted melanoma-associated ME20 antigen, SILV, Silver homolog,gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 26-100kDa

ID gène: 6490, 95972

UniProt: P40967