Recombinant Melanoma gp100 anticorps

N° du produit ABIN6938393

Détail du produit anti-Melanoma gp100 anticorps

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60 64 kDa), which is subsequently cleaved to generate 26 kDa and 34 38 kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

Aucune reactivité croisée: Chien, Rat (Rattus)

Réactivité croisée (Details): Human. Does not react with dog and rat

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human gp100 protein

Clone: PMEL-1825R

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: COLO-38 or SK-MEL-28 cells. Human melanoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: SILV (PMEL Produits)

Synonymes: anticorps D12S53E, anticorps ME20, anticorps ME20-M, anticorps ME20M, anticorps P1, anticorps P100, anticorps PMEL17, anticorps SI, anticorps SIL, anticorps SILV, anticorps gp100, anticorps D10H12S53E, anticorps D12S53Eh, anticorps Pmel17, anticorps Si, anticorps Silv, anticorps gp87, anticorps RPE1, anticorps MMP115, anticorps silverb, anticorps silver, anticorps cb397, anticorps fdv, anticorps pmel17, anticorps sb:cb397, anticorps silva, anticorps wu:fc11g11, anticorps wu:fj24g11, anticorps zgc:136622, anticorps premelanosome protein, anticorps premelanosome protein b, anticorps premelanosome protein a, anticorps PMEL, anticorps Pmel, anticorps pmelb, anticorps pmela

Sujet: 95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanosomal matrix protein17, p100, p26, SILV, Silver homolog,gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)
Cellular localisation: Cytoplasm

Poids moléculaire: 26-100kDa

ID gène: 6490, 95972

UniProt: P40967