Melanoma gp100 anticorps

N° du produit ABIN6938394

L’anticorps anti-Melanoma gp100 Polyclonal Lapin est utilisé pour la détection de Melanoma gp100 dans des échantillons de Humain. Il a été validé pour IHC, WB, IF et FACS.

Aperçu rapide pour Melanoma gp100 anticorps (ABIN6938394)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Melanoma gp100 anticorps

Fonction: gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody

Specificité: The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26 kDa and 34-38 kDa fragments. Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab Purified by Protein A.

Immunogène: Recombinant full-length human SILV protein

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: CACO-38 or SK-MEL-28 cells. Melanoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: SILV

Sujet:

95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

CACO-38 or SK-MEL-28 cells. Melanoma.

Poids moléculaire: 26-100kDa

ID gène: 6490, 95972

UniProt: P40967